Mass Cytometry Core Facility
01 / Helios
Helios is the third generation mass cytometer (CyTOF) by Fluidigm, which is a technology for phenotypic analysis of single cells using inductively coupled plasma time-of-flight mass spectrometry (ICP-MS). Mass cytometry is revolutionizing the ability to analyze single cells in unprecedented depth and breadth with minimal sample sizes. More than 40 markers can be analyzed simultaneously on a single cell. This allows the capture of a tremendous amount of data with a relative low number of cells.
02 / Imaging Mass Cytometer
Imaging mass cytometry is a complementary technology to the Helios system that allows analysis of tissue samples staining with metal-conjugated antibodies to obtain spatial resolution of protein expression at the single-cell level. The tissue is scanned by a small-spot-size pulsed laser and 1micron spot is vaporized on each laser shot and carried into the Helios system. Metal isotopes associated with each spot is measured and indexed against their location in the tissue. This allows the mapping of target proteins throughout the tissue section and the digital reconstruction of the tissue imaging as well as the analysis of single cell parameters in the microenvironment. Tissues can be interrogated with more than 40 parameters simultaneously.
Vinicius Motta, PhD
Tel (514) 398-8203
Goodman Cancer Research Centre
1160 Pine Avenue West, room 401
Montreal, Quebec, Canada
The core facility can sell aliquots of antibodies.
The aim is to provide researches with the option to purchase small amounts of antibodies to analyze few samples or to validate expression of markers of interest. See our antibody list
The core facility can assist on mass cytometry data analysis.
Contact us to discuss your project.
We gratefully acknowledge the financial support from:
Dr. John R. Fraser and
Mrs. Clara M. Fraser