Cell Vision Core Facility: Flow cytometry and single cell analysis
Welcome to the Cell Vision Core Facility for flow cytometry and single cell analysis!
Located in the Bellini pavilion of the Life Science Complex, the Core facility provides instrumentation and technical assistance to perform laser-based flow cytometric analysis, as well as providing an operator based cell sorting service.
The Cell Vision Core Facility operates a four-laser Becton Dickenson(BD)-Aria Fusion cell sorter equipped with a biosafety cabinet, a Miltenyi-AutoMACS Pro cell separator and five cytometers: one four laser BD-LSRFortessa analyzer, one three-laser BD-CantoII, one three-laser Beckman Coulter-Gallios with a 32 tubes carrousel, and a pair of two-laser BD-FACSCalibur analyzers.
We are open Monday through Friday from 9:30 AM until 5:30 PM for cell sorting services and user assistance. The cytometers are accessible after hours for trained users. Training on analyzers are provided by facility staff. Contact us for an instrument training, to schedule a sort or for technical advice on experimental design.
We are located on the third floor of the Bellini Pavilion in the Life Science Complex. The instruments are in rooms 343 and 345.
Note that given current demand for cell sorting appointments, we recommend that you book your sort at least 1.5 - 2 weeks ahead of time.
Acknowledging core support
All users and investigators can help us maintain our excellence by acknowledging core support. When preparing manuscripts that contain work originating from services or resources provided by the Cell Vision Core Facility, please acknowledge support.
Here is a suggested acknowledgment:
“The flow cytometry work/ cell sorting was performed in the Cell Vision Core Facility for flow cytometry and single cell analysis of the Life Science Complex and supported by funding from the Canadian Foundation for Innovation.”
Please inform us of each publication acknowledging our core to help us combine and summarize them for grant application purposes.
The Cell Vision Core Facility would like to thank the Canadian Foundation for Innovation. We receive considerable financial aid through the grants awarded to the Rosalind and Morris Goodman Cancer Centre, the Complex Traits Group, and the Life Science Complex in general. Without this support, the purchase and maintenance of our high-end equipment as well as the defrayal of the high operating costs of the facility wouldn’t be possible.